Phospholipase C Expression and Activity in Smooth Muscle Cells of Renal Arterioles and Aorta of Young, Spontaneously Hypertensive Rats During Culture | Zendy

Z PENG | Zendy; William J. Arendshorst | Zendy

Open Access

Phospholipase C Expression and Activity in Smooth Muscle Cells of Renal Arterioles and Aorta of Young, Spontaneously Hypertensive Rats During Culture

Author(s) -

Z PENG,

William J. Arendshorst

Publication year - 2007

Publication title -

american journal of hypertension

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.009

H-Index - 136

eISSN - 1941-7225

pISSN - 0895-7061

DOI - 10.1016/j.amjhyper.2006.12.014

Subject(s) - vascular smooth muscle , isozyme , aorta , medicine , endocrinology , phospholipase c , western blot , phospholipase , enzyme , blood vessel , inositol , biology , biochemistry , receptor , smooth muscle , gene

Phospholipase C (PLC)-beta(1) and -delta(1), but not -gamma(1), protein expressions in fresh renal arterioles and aorta are greater in 6-week-old, spontaneously hypertensive rats (SHRs) versus normotensive Wistar-Kyoto rats (WKYs). This PLC activity is also greater in both vessels of SHRs. In the present study, we tested whether cultured vascular smooth muscle cells (VSMCs) of preglomerular arterioles and aorta accurately reflect strain differences observed in fresh vessels, with VSMCs of SHRs predicted to have higher levels of PLC isozymes and enzyme activity. We assessed the stability of variables over passages 3 to 11.

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