Association of C825T polymorphism of the G protein β3-subunit with antihypertensive response to a thiazide diuretic

Objective: To investigate the association of T allele of the C825T polymorphism of the gene encoding the 3-subunit of G proteins (GNB3) with blood pressure response to diuretic therapy. Methods: Polymerase chain reaction combined with restriction enzyme digestion was used to detect the polymorphism of G protein of b3 subunit gene in 147 normotensive controls and 321hypertensive patients.48 hypertensive patients underwent monotherapy with hydrochlorothiazide for 4 weeks. Results: Mean declines in systolic and diastolic blood pressures were significantly greater in CT heterozygotes and TT homozygous (22.67 13.74mmHg,12.00 9.39mmHg) than in CC homozygotes(8.81 6.23 mmHg, 6.50 5.32 mmHg).In multiple regression models, the TT genotype remained a significant predictor of greater declines in systolic and diastolic blood pressures,(p 0.05). There were no significant difference of the GNB3 gene C825T polymorphism genotypes and alleles between hypertensive patients (CC:28.7%,CT:52%,TT:19.3% and C:50.7%,T:49.3%) and normotensive controls (CC:27.2%,CT:46.9%,TT: 25.9% and C:50.7%,T:49.3%). Conclusions: The C825T polymorphism of GNB3 may help identify patients with essential hypertension who are more responsive to hydrochlorothiazide therapy,but the C825T polymorphism of GNB3 is not associated with essential hypertension.