Whole mount in situ hybridization and immunohistochemistry for studying retinoic acid signaling in developing amphioxus | Zendy

Matteo Bozzo | Zendy; Simona Candiani | Zendy; Michael Schubert | Zendy

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Whole mount in situ hybridization and immunohistochemistry for studying retinoic acid signaling in developing amphioxus

Author(s) -

Matteo Bozzo,

Simona Candiani,

Michael Schubert

Publication year - 2020

Publication title -

methods in enzymology on cd-rom/methods in enzymology

Language(s) - English

Resource type - Journals

eISSN - 1079-2376

pISSN - 0076-6879

DOI - 10.1016/bs.mie.2020.03.007

Subject(s) - retinoic acid , chordate , in situ hybridization , biology , vertebrate , immunohistochemistry , microbiology and biotechnology , anatomy , immunology , genetics , cell culture , gene , gene expression

Retinoic acid (RA) is a vitamin A-derived signaling molecule acting during development and in the adult. This chapter provides protocols to characterize the role of RA signaling during development of the invertebrate chordate amphioxus. As sister group to all other chordates and characterized by the most vertebrate-like RA signaling system of all invertebrates, amphioxus is an important model for studying the evolution of RA signaling. Focusing on the development of GABAergic neurons in the amphioxus central nervous system, we provide detailed protocols for maintaining and breeding adult animals, for performing pharmacological treatments of embryos and for analyzing the effects of these treatments by whole mount in situ hybridization and immunohistochemistry coupled to confocal microscopy.

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