Open AccessMatrix design for matrix-assisted laser desorption ionization: Sensitive determination of PAH-DNA adducts
Author(s) -
M. C. George,
Joelle M. Y. Wellemans,
Ronald L. Cerny,
Michael L. Gross,
K. Li,
Ercole L. Cavalieri
Publication year - 1994
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/1044-0305(94)80021-9
Subject(s) - chemistry , adduct , desorption , analyte , matrix (chemical analysis) , polycyclic aromatic hydrocarbon , matrix assisted laser desorption/ionization , ionization , dna , soft laser desorption , mass spectrometry , analytical chemistry (journal) , hydrocarbon , matrix assisted laser desorption electrospray ionization , atmospheric pressure laser ionization , chromatography , organic chemistry , electron ionization , biochemistry , ion , adsorption
Two matrices, 4-phenyl-α-eyanocinnamic acid (PCC) and 4-benzyloxy-α-eyanocinnamic acid (BCC), were identified for the determination of polycyclic aromatic hydrocarbon (PAH) adducts of DNA bases by matrix-assisted laser desorption ionization. These matrices were designed based on the concept that the matrix and the analyte should have structural similarities. PCC is a good matrix for the desorption of not only PAH-modified DNA bases, but also PAHs themselves and their metabolites. Detections can be made at the femtomolar level.