Open AccessAmino acid sequence prerequisites for the formation of cn ions
Author(s) -
Kevin M. Downard,
K. Biemann
Publication year - 1993
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/1044-0305(93)87005-w
Subject(s) - chemistry , cleavage (geology) , threonine , tryptophan , serine , ion , fragmentation (computing) , amino acid , stereochemistry , lysine , amino acid residue , peptide sequence , biochemistry , organic chemistry , phosphorylation , geotechnical engineering , fracture (geology) , computer science , engineering , gene , operating system
Ammo acid sequence prerequisites are described for the formation of c, ions observed in high-energy collision-induced decomposition spectra of peptides. It is shown that the formation of cn ions is promoted by the nature of the amino acid C-terminal to the cleavage site. A propensity for cn cleavage preceding threonine, and to a lesser extent tryptophan, lysine, and serine, is demonstrated where fragmentation is directed N-terminally at these residues. In addition, the nature of the residue N-terminal to the cleavage site is shown to have little effect on cn ion formation. A mechanism for cn ion formation is proposed and its applicability to the results observed is discussed.