Open AccessIon cloud manipulation using the radiofrequency-only-mode as an improvement for high mass detection in fourier transform mass spectrometry
Author(s) -
Cliffton B. Jacoby,
Christopher L. Holliman,
Don L. Rempel,
Michael L. Gross
Publication year - 1993
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1016/1044-0305(93)85076-a
Subject(s) - chemistry , mass spectrometry , analytical chemistry (journal) , ion , ion trap , fourier transform ion cyclotron resonance , fourier transform , physics , chromatography , organic chemistry , quantum mechanics
It has been difficult to achieve the expected high resolving power for high-mass biomolecule ions in Fourier transform mass spectrometry. Our hypothesis is that ion clouds produced by laser desorption or injection are diffuse and produce poor signals. To test the hypothesis, clouds of benzene molecular ions produced by electron ionization were purposefully expanded via magnetron mode excitation and characterized by a new experimental sequence for cloud sectional analysis. The expanded cloud was then successfully focused to the trap center by using a high-pressure dynamic event (radiofrequency-only mode). The expanded cloud in a conventional cubic trap produces no detectable signal, whereas the focused cloud in a compensated trap yields a high-resolution signal with good signal-to-noise ratio.