Open AccessCellbow: a robust customizable cell segmentation program
Author(s) -
Ren Huixia,
Zhao Mengdi,
Liu Bo,
Yao Ruixiao,
Liu Qi,
Ren Zhipeng,
Wu Zirui,
Gao Zongmao,
Yang Xiaojing,
Tang Chao
Publication year - 2020
Publication title -
quantitative biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.707
H-Index - 15
eISSN - 2095-4697
pISSN - 2095-4689
DOI - 10.1007/s40484-020-0213-6
Subject(s) - segmentation , computer science , robustness (evolution) , artificial intelligence , image segmentation , population , computer vision , software , market segmentation , visualization , process (computing) , pattern recognition (psychology) , biochemistry , chemistry , demography , marketing , sociology , business , gene , programming language , operating system
Background Time‐lapse live cell imaging of a growing cell population is routine in many biological investigations. A major challenge in imaging analysis is accurate segmentation, a process to define the boundaries of cells based on raw image data. Current segmentation methods relying on single boundary features have problems in robustness when dealing with inhomogeneous foci which invariably happens in cell population imaging. Methods Combined with a multi‐layer training set strategy, we developed a neural‐network‐based algorithm — Cellbow. Results Cellbow can achieve accurate and robust segmentation of cells in broad and general settings. It can also facilitate long‐term tracking of cell growth and division. To facilitate the application of Cellbow, we provide a website on which one can online test the software, as well as an ImageJ plugin for the user to visualize the performance before software installation. Conclusion Cellbow is customizable and generalizable. It is broadly applicable to segmenting fluorescent images of diverse cell types with no further training needed. For bright‐field images, only a small set of sample images of the specific cell type from the user may be needed for training.