Genetic characterization of Lithuanian honeybee lines based on ISSR polymorphism | Zendy

Violeta Čeksterytė | Zendy; V. Paplauskienė | Zendy; Diana Tamašauskienė | Zendy; I. Pašakinskienė | Zendy; Ingrida Mažeikienė | Zendy

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Genetic characterization of Lithuanian honeybee lines based on ISSR polymorphism

Author(s) -

Violeta Čeksterytė,

V. Paplauskienė,

Diana Tamašauskienė,

I. Pašakinskienė,

Ingrida Mažeikienė

Publication year - 2012

Publication title -

apidologie

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.811

H-Index - 83

eISSN - 1297-9678

pISSN - 0044-8435

DOI - 10.1007/s13592-012-0140-2

Subject(s) - subspecies , biology , dendrogram , phylogenetic tree , hybrid , genetics , dna profiling , genetic marker , polymorphism (computer science) , genetic distance , genotype , genetic diversity , genetic variation , zoology , botany , dna , gene , population , demography , sociology

International audienceThis study presents the first results from the selection and evaluation of inter-simple sequence repeat markers for the genetic assessment of honeybee lines developed in Lithuania and introduced subspecies. Two Lithuania-bred lines of Apis mellifera carnica were compared to those introduced from Czech Republic and Slovenia and also to a subspecies introduced from the Caucasus (Apis mellifera caucasica) and local Buckfast hybrids. The genetic constitution was assayed with four simple sequence primers: (ATG)5GA, (TCC)5GT, (AGAC)4GC and (GACA)4GT. Trinucleotide motif primers used in the bee DNA assays produced five to 10 fragments and tetranucleotide ones, six to seven. Primer (TCC)5GT generated a distinct A. m. caucasica-specific 800-bp fragment, which was also present in 40 % of the Buckfast individuals. A dendrogram based on the UPGMS method generated four sub-clusters. One of the Lithuania-bred A. m. carnica lines clustered apart from the other A. m. carnica lines. In a rooted phylogenetic tree, the group containing A. m. caucasica and Buckfast hybrids appeared well differentiated from the A. m. carnica lines

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