Open AccessMatrix Pre-Coated MALDI MS Targets for Small Molecule Imaging in Tissues
Author(s) -
Kerri Grove,
Sara L. Frappier,
Richard M. Caprioli
Publication year - 2011
Publication title -
journal of the american society for mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 127
eISSN - 1879-1123
pISSN - 1044-0305
DOI - 10.1007/s13361-010-0013-8
Subject(s) - chemistry , analyte , maldi imaging , matrix (chemical analysis) , sample preparation , matrix assisted laser desorption/ionization , small molecule , complex matrix , mass spectrometry , mass spectrometry imaging , coating , chromatography , nanotechnology , biochemistry , organic chemistry , materials science , adsorption , desorption
A new sample preparation method for MALDI tissue imaging has been developed for the analysis of low molecular weight compounds that employs matrix pre-coated MALDI targets. Tissue sections need only to be transferred onto the pre-coated target before analysis for fast and easy sample preparation. Pre-coated targets have a homogenous matrix coating with uniform crystals of approximately 1-2 μm and do not require solvents that may lead to analyte delocalization within a tissue section. We report here the use of matrix pre-coated targets for imaging of lipids, peptides, and pharmaceuticals in tissues.