Open AccessThe analysis of correlation between IL-12 gene expression and hepatitis B virus in the affected patients
Author(s) -
Abdolhossein Zare,
Ahmad Rashki,
Sajjad Ghahari,
Bashir Ghayoori
Publication year - 2015
Publication title -
virusdisease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.375
H-Index - 22
eISSN - 2347-3517
pISSN - 2347-3584
DOI - 10.1007/s13337-015-0261-0
Subject(s) - hepatitis b virus , hepatocellular carcinoma , cirrhosis , sybr green i , hepatitis b , immunology , immune system , medicine , virus , immunity , gene expression , virology , cytokine , gene , real time polymerase chain reaction , biology , biochemistry
Hepatitis B viral (HBV) infection, which is one of the global public health concerns, is among the most common causes of cirrhosis and hepatocellular carcinoma. It was proposed that cytokine-mediated immunity plays a critical role in determining the outcomes of hepatitis B virus infection. Interleukin 12 (IL-12) is a heterodimeric cytokine that shows a broad range of immunoregulatory properties during immune responses and combats host invading pathogens. The main purpose of this study was to investigate the possible association between expression levels of IL-12 gene with HBV infection in patients with HBV infection. This clinical study was performed on 30 HBV patients and 30 healthy controls. SYBR Green Real-time PCR was performed to examine the expression level of IL-12 gene in HBV patients. Then, the rate of expression was calculated using the Livak ([Formula: see text] ) method. ΔCT of samples in the two groups were compared using t test method. PCR was also used for HBV-DNA evidence. The results of our study demonstrated that the difference in mean of IL-12 gene expression between healthy subjects and HBV patients is statistically significant.