Microsatellite markers for Australian temperate diadromous fishes Pseudaphritis urvillii (Bovichtidae) and Lovettia sealii (Galaxiidae) | Zendy

Daniel J. Schmidt | Zendy; Kathryn M. Real | Zendy; David A. Crook | Zendy; Jane M. Hughes | Zendy

Open Access

Microsatellite markers for Australian temperate diadromous fishes Pseudaphritis urvillii (Bovichtidae) and Lovettia sealii (Galaxiidae)

Author(s) -

Daniel J. Schmidt,

Kathryn M. Real,

David A. Crook,

Jane M. Hughes

Publication year - 2012

Publication title -

conservation genetics resources

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.353

H-Index - 23

eISSN - 1877-7260

pISSN - 1877-7252

DOI - 10.1007/s12686-012-9800-9

Subject(s) - biology , loss of heterozygosity , fish migration , microsatellite , locus (genetics) , allele , genetics , temperate climate , evolutionary biology , zoology , ecology , gene , habitat

Thirteen microsatellite loci were developed and characterised for two fishes from temperate Australia that exhibit atypical diadromous migration strategies. Cloning and sequencing of an enriched partial genomic library was used to develop seven highly polymorphic loci for the catadromous species Pseudaphritis urvillii (known as tupong or congolli). Mean number of alleles per locus was 16.5, and average observed and expected heterozygosity was between 0.90 and 0.87, respectively. Six polymorphic markers characterised for the anadromous species Lovettia sealii (known as Tasmanian whitebait) included a mean of 12.3 alleles per locus and average observed and expected heterozygosity of 0.71-0.77, respectively. These microsatellites will be employed to understand regional patterns of recruitment, migration and stock structure.Griffith Sciences, Griffith School of EnvironmentFull Tex

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