Characterization of microsatellite loci in the straw-colored fruit bat, Eidolon helvum (Pteropodidae) | Zendy

Alison J. Peel | Zendy; Stephen J. Rossiter | Zendy; James L. N. Wood | Zendy; Andrew A. Cunningham | Zendy; David R. Sargan | Zendy

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Characterization of microsatellite loci in the straw-colored fruit bat, Eidolon helvum (Pteropodidae)

Author(s) -

Alison J. Peel,

Stephen J. Rossiter,

James L. N. Wood,

Andrew A. Cunningham,

David R. Sargan

Publication year - 2010

Publication title -

conservation genetics resources

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.353

H-Index - 23

eISSN - 1877-7260

pISSN - 1877-7252

DOI - 10.1007/s12686-010-9190-9

Subject(s) - biology , microsatellite , loss of heterozygosity , locus (genetics) , gene flow , allele , mitochondrial dna , genetics , evolutionary biology , gene , genetic variation

Twenty microsatellite loci developed for the straw-colored fruit bat, Eidolon helvum, are described. These markers were used in multiplex PCRs to amplify genomic DNA from 142 individuals sampled from nine populations across Africa. Nineteen loci were polymorphic, with a mean number of alleles per locus of 16.2 (3-46). Observed and expected heterozygosity values ranged from 0.01 to 0.90 and 0.03 to 0.97, respectively. These markers will be used in combination with mitochondrial DNA sequences to investigate gene flow and the genetic metapopulation structure of E. helvum.Full Tex

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