Open AccessChronic shisha exposure alters phosphoproteome of oral keratinocytes
Author(s) -
Patil Shankargouda,
Rajagopalan Pavithra,
Patel Krishna,
Subbannayya Tejaswini,
Babu Niraj,
Mohan Sonali V.,
Advani Jayshree,
Sathe Gajanan,
Bhandi Shilpa,
Solanki Hitendra S.,
Sidransky David,
Chatterjee Aditi,
Gowda Harsha,
Ferrari Marco
Publication year - 2019
Publication title -
journal of cell communication and signaling
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 44
eISSN - 1873-961X
pISSN - 1873-9601
DOI - 10.1007/s12079-019-00528-4
Subject(s) - downregulation and upregulation , immunology , medicine , proteome , biology , gene , bioinformatics , genetics
Shisha smoking has been epidemiologically linked to oral cancer. However, few studies have investigated the pathobiology of shisha‐induced cellular transformation. We studied the effects of chronic shisha exposure (8 months) in an in vitro model using immortalized, non‐neoplastic oral keratinocytes (OKF6/TERT1). Quantitative proteomic and phosphoproteomic analyses were performed on OKF6/TERT1 cells treated with shisha extract for a period of 8 months. Pathway analysis was carried out to identify significantly enriched biological processes in shisha‐treated cells. Chronic shisha exposure resulted in increased cell scattering phenomenon in OKF6/TERT1 cells. Data analysis revealed differential phosphorylation of 164 peptides (fold change ≥1.5, p ≤ 0.0.5) corresponding to 136 proteins. Proteins associated with mTORC1 and EIF4F complexes involved in initiating protein translation were seen to be enriched upon shisha treatment. Network analysis also highlighted downregulation of proteins involved in Type I interferon signaling in shisha‐treated cells. Quantitative phosphoproteomic approach elucidated global perturbations to the molecular milieu of oral keratinocytes upon shisha exposure. Further studies are needed to validate putative targets in oral cancer patients with shisha smoking history.