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Possible reparative effect of low‐intensity pulsed ultrasound (LIPUS) on injured meniscus
Author(s) -
Kamatsuki Yusuke,
Aoyama Eriko,
Furumatsu Takayuki,
Miyazawa Shinichi,
Maehara Ami,
Yamanaka Nobuyasu,
Nishida Takashi,
Kubota Satoshi,
Ozaki Toshifumi,
Takigawa Masaharu
Publication year - 2018
Publication title -
journal of cell communication and signaling
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 44
eISSN - 1873-961X
pISSN - 1873-9601
DOI - 10.1007/s12079-018-0496-9
Subject(s) - aggrecan , low intensity pulsed ultrasound , meniscus , cartilage , fibrocartilage , extracellular matrix , wound healing , type ii collagen , medicine , osteoarthritis , cartilage oligomeric matrix protein , pathology , microbiology and biotechnology , anatomy , surgery , ultrasound , articular cartilage , therapeutic ultrasound , biology , physics , alternative medicine , incidence (geometry) , optics , radiology
Abstract Menisci are a pair of crescent‐shaped fibrocartilages, particularly of which their inner region of meniscus is an avascular tissue. It has characteristics similar to those of articular cartilage, and hence is inferior in healing. We previously reported that low‐intensity pulsed ultrasound (LIPUS) treatment stimulates the production of CCN2/CTGF, a protein involved in repairing articular cartilage, and the gene expression of major cartilage matrices such as type II collagen and aggrecan in cultured chondrocytes. Therefore, in this present study, we investigated whether LIPUS has also favorable effect on meniscus cells and tissues. LIPUS applied with a 60 mW/cm 2 intensity for 20 min stimulated the gene expression and protein production of CCN2 via ERK and p38 signaling pathways, as well as gene expression of SOX9, aggrecan, and collagen type II in human inner meniscus cells in culture, and slightly stimulated the gene expression of CCN2 and promoted the migration in human outer meniscus cells in culture. LIPUS also induced the expression of Ccn2, Sox9, Col2a1, and Vegf in rat intact meniscus. Furthermore, histological evaluations showed that LIPUS treatment for 1 to 4 weeks promoted healing of rat injured lateral meniscus, as evidenced by better and earlier angiogenesis and extracellular matrix synthesis. The data presented indicate that LIPUS treatment might prevent meniscus from degenerative change and exert a reparative effect on injured meniscus via up‐regulation of repairing factors such as CCN2 and that it might thus be useful for treatment of an injured meniscus as a non‐invasive therapy.

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