Open AccessCCN2/CTGF binds the small leucine rich proteoglycan protein Tsukushi
Author(s) -
Ohta Kunimasa,
Aoyama Eriko,
Ahmad Shah Adil Ishtiyaq,
Ito Naofumi,
Anam Mohammad Badrul,
Kubota Satoshi,
Takigawa Masaharu
Publication year - 2018
Publication title -
journal of cell communication and signaling
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 44
eISSN - 1873-961X
pISSN - 1873-9601
DOI - 10.1007/s12079-018-0487-x
Subject(s) - ctgf , aggrecan , microbiology and biotechnology , proteoglycan , extracellular matrix , cartilage , adamts , signal transduction , chemistry , fibronectin , bone morphogenetic protein , biology , thrombospondin , growth factor , biochemistry , anatomy , metalloproteinase , matrix metalloproteinase , receptor , medicine , pathology , osteoarthritis , gene , alternative medicine , articular cartilage
Extracellular molecules coordinate the multiple signaling pathways spatiotemporally to exchange information between cells during development. Understanding the regulation of these signal molecule‐dependent pathways elucidates the mechanism of intercellular crosstalks. CCN2/CTGF is one of the CCN family members that binds BMP2, fibronectin, aggrecan, FGFR2 ‐ regulating cartilage and bone formation, angiogenesis, wound repair etc. Tsukushi (TSK), which belongs to the Small Leucine‐Rich Proteoglycan (SLRP) family, binds nodal/Vg1/TGF‐β1, BMP4/chordin, Delta, FGF8, Frizzled4, and is involved in the early body formation, bone growth, wound healing, retinal stem cell regulation etc. These two secreted molecules are expressed in similar tissues and involved in several biological events by functioning as extracellular signaling modulators. Here, we examine the molecular interaction between CCN2 and TSK biochemically. Co‐precipitation assay and Surface Plasmon Resonance measurement showed their direct binding with the Kd value 15.3 nM. Further, the Solid‐phase Binding Assay indicated that TSK binds to IGFBP and CT domains of CCN2. Our data suggest that CCN2 and TSK exert their function together in the body formation.