Open Accessp38 differentially regulates ERK, p21, and mitogenic signalling in two pancreatic carcinoma cell lines
Author(s) -
Aasrum Monica,
Thoresen G. Hege,
Christoffersen Thoralf,
Brusevold Ingvild J.
Publication year - 2018
Publication title -
journal of cell communication and signaling
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 44
eISSN - 1873-961X
pISSN - 1873-9601
DOI - 10.1007/s12079-017-0444-0
Subject(s) - mapk/erk pathway , dna synthesis , mek inhibitor , downregulation and upregulation , kinase , p38 mitogen activated protein kinases , cell growth , cell culture , microbiology and biotechnology , cancer research , epidermal growth factor , biology , mitogen activated protein kinase , chemistry , dna , biochemistry , genetics , gene
Whereas the p38 MAP kinase has largely been associated with anti‐proliferative functions, several observations have indicated that it may also have positive effects on proliferation. In hepatocytes, we have found that p38 has opposing effects on DNA synthesis when activated by EGF and HGF. Here we have studied the function of p38 in EGF‐ and HGF‐induced DNA synthesis in the two pancreatic carcinoma cell lines AsPC‐1 and Panc‐1. In Panc‐1 cells, the MEK inhibitor PD98059 reduced EGF‐ and HGF‐induced DNA synthesis, while the p38 inhibitor SB203580 strongly increased the basal DNA synthesis and reduced expression of the cyclin‐dependent kinase inhibitor (CDKI) p21. In contrast, in AsPC‐1 cells, EGF‐ and HGF‐induced DNA synthesis was not significantly reduced by PD98059 but was inhibited by SB203580. Treatment with SB203580 amplified the sustained ERK phosphorylation induced by these growth factors and caused a marked upregulation of the expression of p21, which could be blocked by PD98059. These results suggest that while DNA synthesis in Panc‐1 cells is enhanced by ERK and strongly suppressed by p38, in AsPC‐1 cells, p38 exerts a pro‐mitogenic effect through MEK/ERK‐dependent downregulation of p21. Thus, p38 may have suppressive or stimulatory effects on proliferation depending on the cell type, due to differential cross‐talk between the p38 and MEK/ERK pathways.