Open Access1,25(OH)2 vitamin D3 signalling on immature rat Sertoli cells: gamma‐glutamyl transpeptidase and glucose metabolism
Author(s) -
Gonçalves Renata,
Zamoner Ariane,
Zanatta Leila,
Zanatta Ana Paula,
Remor Aline Pertile,
Luz Scheffer Débora,
Latini Alexandra,
Silva Fátima Regina Mena Barreto
Publication year - 2017
Publication title -
journal of cell communication and signaling
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 44
eISSN - 1873-961X
pISSN - 1873-9601
DOI - 10.1007/s12079-016-0367-1
Subject(s) - sertoli cell , medicine , endocrinology , spermatogenesis , biology , metabolism , vitamin , reactive oxygen species , glutathione , chemistry , biochemistry , enzyme
1α,25‐Dihydroxyvitamin D 3 (1,25‐D 3 ) is critical for the maintenance of normal male reproduction since reduced fertility is observed in vitamin D‐deficient rats. Gamma‐glutamyl transpeptidase (GGT) is a membrane‐bound enzyme that is localized on Sertoli cells and catalyses the transfer of the gamma‐glutamyl residues to an amino acid or peptide acceptor. Sertoli cells are also responsible for providing nutrients, as lactate, to the development of germ cells. The aim of this study was to investigate the effect and the mechanism of action of 1,25‐D 3 on GGT on Sertoli cell functions from 30‐day‐old immature rat testis. Results demonstrated that 1,25‐D 3 stimulates GGT activity at Sertoli cells plasma membrane through a PKA‐dependent mechanism of action, which was not dependent of active de novo protein synthesis. The hormone increases glucose uptake, as well as lactate production and release by Sertoli cells without altering the reactive oxygen species (ROS) generation. In addition, 1,25‐D 3 did not change reduced glutathione (GSH) amount or oxygen consumption, and diminished Sertoli cell death. These findings demonstrate that 1,25‐D 3 stimulatory effect on GGT activity, glucose uptake, LDH activity and lactate production seem to be an important contribution of Sertoli cells for germ cells nutrition and for a full and active ongoing spermatogenesis.