Open AccessDifferent expression and subcellular localization of Phosphoinositide‐specific Phospholipase C enzymes in differently polarized macrophages
Author(s) -
Di Raimo Tania,
Leopizzi Martina,
Mangino Giorgio,
Rocca Carlo Della,
Businaro Rita,
Longo Lucia,
Lo Vasco Vincenza Rita
Publication year - 2016
Publication title -
journal of cell communication and signaling
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 44
eISSN - 1873-961X
pISSN - 1873-9601
DOI - 10.1007/s12079-016-0335-9
Subject(s) - subcellular localization , microbiology and biotechnology , enzyme , signal transduction , biology , gene isoform , gene expression , phospholipase c , phenotype , macrophage polarization , phosphoinositide phospholipase c , inflammation , gene , biochemistry , cytoplasm , immunology
Macrophages’ phenotypic and functional diversity depends on differentiating programs related to local environmental factors. Recent interest was deserved to the signal transduction pathways acting in macrophage polarization, including the phosphoinositide (PI) system and related phospholipase C (PLC) family of enzymes. The expression panel of PLCs and the subcellular localization differs in quiescent cells compared to the pathological counterpart. We analyzed the expression of PLC enzymes in unpolarized (M0), as well as in M1 and M2 macrophages to list the expressed isoforms and their subcellular localization. Furthermore, we investigated whether inflammatory stimulation modified the basal panel of PLCs’ expression and subcellular localization. All PLC enzymes were detected within both M1 and M2 cells, but not in M0 cells. M0, as well as M1 and M2 cells own a specific panel of expression, different for both genes’ mRNA expression and intracellular localization of PLC enzymes. The panel of PLC genes’ expression and PLC proteins’ presence slightly changes after inflammatory stimulation. PLC enzymes might play a complex role in macrophages during inflammation and probably also during polarization.