Open AccessDiabetes‐induced fibrotic matrix inhibits intramembranous bone healing
Author(s) -
Khosravi Roozbeh,
Trackman Philip C.
Publication year - 2015
Publication title -
journal of cell communication and signaling
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 44
eISSN - 1873-961X
pISSN - 1873-9601
DOI - 10.1007/s12079-014-0242-x
Subject(s) - intramembranous ossification , bone healing , calvaria , medicine , ossification , endochondral ossification , extracellular matrix , bone remodeling , diabetes mellitus , endocrinology , pathology , microbiology and biotechnology , anatomy , cartilage , chemistry , biology , in vitro , biochemistry
Diabetes diminishes bone healing and ossification. Reduced bone formation in intramembranous ossification is known, yet the mechanism(s) behind impaired intramembranous bone healing are unclear. Here we report the formation of a fibrotic matrix during healing of intramembranous calvarial bone defects that appears to exclude new bone growth. Our histological analyses of 7‐day and 14‐day calvaria bone healing tissue in chemically‐induced diabetic mice and non‐diabetic mice showed the accumulation of a non‐mineralized fibrotic matrix, likely as a consequence of unresolved hematomas under diabetic conditions. Elevated mRNA and enzyme activity levels of lysyl oxidase on day 7 in diabetic bone healing tissues also supports that the formation of a fibrotic matrix occurs in these tissues. Based on these findings, we propose that elevated fibroblast proliferation and formation of a non‐mineralized fibrotic extracellular matrix in diabetes contributes to deficient intramembranous bone healing in diabetes. A greater understanding of this process has relevance to managing dental procedures in diabetics in which successful outcomes depend on intramembranous bone formation.