Open AccessDirect interaction between CCN family protein 2 and fibroblast growth factor 1
Author(s) -
Abd El Kader Tarek,
Kubota Satoshi,
Anno Ken,
Tanaka Saho,
Nishida Takashi,
Furumatsu Takayuki,
Aoyama Eriko,
Kuboki Takuo,
Takigawa Masaharu
Publication year - 2014
Publication title -
journal of cell communication and signaling
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 44
eISSN - 1873-961X
pISSN - 1873-9601
DOI - 10.1007/s12079-014-0232-z
Subject(s) - cartilage , matricellular protein , fibroblast growth factor , microbiology and biotechnology , fibroblast , chemistry , ctgf , interactome , growth factor , biology , extracellular matrix , anatomy , biochemistry , gene , in vitro , receptor
In an attempt to find out a new molecular counterpart of CCN family protein 2 (CCN2), a matricellular protein with multiple functions, we performed an interactome analysis and found fibroblast growth factor (FGF) ‐1 as one of the candidates. Solid‐phase binding assay indicated specific binding between CCN2 and FGF‐1. This binding was also confirmed by surface plasmon resonance (SPR) analysis that revealed a dissociation constant (Kd) of 3.98 nM indicating strong molecular interaction between the two. RNA analysis suggested that both FGF‐1 and CCN2 could be produced by chondrocytes and thus their interaction in the cartilage is possible. These findings for the first time indicate the direct interaction of CCN2 and FGF‐1 and suggest the co‐presence of these molecules in the cartilage microenvironment. CCN2 is a well‐known promoter of cartilage development and regeneration, whereas the physiological and pathological role of FGF‐1 in cartilage mostly remains unclear. Biological role of FGF‐1 itself in cartilage is also suspected.