Open AccessCeramide inhibits CCN2 expression in fibroblasts
Author(s) -
Kennedy Laura,
Parapuram Sunil,
Greenspoon Jill,
Leask Andrew
Publication year - 2008
Publication title -
journal of cell communication and signaling
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 44
eISSN - 1873-961X
pISSN - 1873-9601
DOI - 10.1007/s12079-008-0024-4
Subject(s) - ceramide , smad , ctgf , transforming growth factor , microbiology and biotechnology , fibroblast , luciferase , connective tissue , growth factor , chemistry , biology , apoptosis , transfection , biochemistry , gene , receptor , in vitro , genetics
Connective tissue growth factor (CTGF, CCN2) is induced in response to TGFβ in fibroblasts. In this report, we show that C2 ceramide reduced the ability of TGFβ to induce CCN2 protein, mRNA and promoter activity in fibroblasts. C2 ceramide reduced the ability of TGFβ to induce the generic Smad responsive promoter/reporter construct SBE‐luciferase. These results suggest that C2 ceramide reduces the action of TGFβ in fibroblasts via Smad antagonism.