Open AccessMorphology and growth of mammalian cells in a liquid/liquid culture system supported with oxygenated perfluorodecalin
Author(s) -
Maciej Pilarek,
Iwona Grabowska,
Maria A. Ciemerych,
K. Dąbkowska,
Krzysztof W. Szewczyk
Publication year - 2013
Publication title -
biotechnology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.548
H-Index - 107
eISSN - 1573-6776
pISSN - 0141-5492
DOI - 10.1007/s10529-013-1218-2
Subject(s) - c2c12 , cell culture , multicellular organism , liquid medium , liquid culture , baby hamster kidney cell , morphology (biology) , chemistry , microbiology and biotechnology , biophysics , biochemistry , biology , cell , in vitro , chromatography , myogenesis , genetics , botany
Adherent A431, BHK-21, and C2C12 cells were cultured on a flexible interface formed between two immiscible liquid phases: (i) hydrophobic perfluorodecalin (PFD) and (ii) aqueous culture medium (DMEM). BHK-21 cells formed multicellular aggregates characterized by irregular shapes. A431, as well as C2C12 cells, grew as tight multicellular sheets of 3-D cells. Enhanced mass transfer and facilitated access of the cells to the O2 dissolved in PFD/DMEM by approx. 250 % and thereby increased the density of BHK-21 cells. Thus the liquid/liquid system is a simple, ready-to-use, and fully scalable (independent of vessel shapes); consequently it is a method for 3-D cultures of adherent animal cells in which the growth of anchorage-dependent cells is not limited by confluence effect.