Open AccessHairy Root Cultures of Gynostemma pentaphyllum (Thunb.) Makino: A Promising Approach for the Production of Gypenosides as an Alternative of Ginseng Saponins
Author(s) -
Chen-Kai Chang,
KuShang Chang,
Yichen Lin,
Sin-Yie Liu,
ChienYuan Chen
Publication year - 2005
Publication title -
biotechnology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.548
H-Index - 107
eISSN - 1573-6776
pISSN - 0141-5492
DOI - 10.1007/s10529-005-8653-7
Subject(s) - gynostemma pentaphyllum , ginseng , traditional medicine , agrobacterium , saponin , botany , chemistry , araliaceae , biology , transformation (genetics) , medicine , extraction (chemistry) , chromatography , biochemistry , alternative medicine , pathology , gene
Hairy root cultures of Gynostemma pentaphyllum were established by infecting leaf discs with Agrobacterium rhizogenes. The dry biomass of hairy roots grown in MS medium for 49 days was 7.3 g l(-1) with a gypenoside content of 38 mg g(-1) dry wt.
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