Repurposing the Hybrid Capture 2 (HC2) screening test for whole-genome sequencing of human papillomaviruses | Zendy

Alice Debernardi | Zendy; William Jarassier | Zendy; Christine Soret | Zendy; Christiane Mougin | Zendy; Samuel Alizon | Zendy; Ignacio G. Bravo | Zendy; David Guenat | Zendy; JeanLuc Prétet | Zendy; Quentin Lepiller | Zendy

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Repurposing the Hybrid Capture 2 (HC2) screening test for whole-genome sequencing of human papillomaviruses

Author(s) -

Alice Debernardi,

William Jarassier,

Christine Soret,

Christiane Mougin,

Samuel Alizon,

Ignacio G. Bravo,

David Guenat,

JeanLuc Prétet,

Quentin Lepiller

Publication year - 2021

Publication title -

archives of virology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.943

H-Index - 110

eISSN - 1432-8798

pISSN - 0304-8608

DOI - 10.1007/s00705-021-05259-9

Subject(s) - genotyping , biology , dna sequencing , human papillomavirus , genotype , virology , genome , papillomaviridae , computational biology , deep sequencing , genetics , gene , cervical cancer , medicine , cancer

Simple and standardized approaches for genome analysis of human papillomavirus (HPV) by next-generation sequencing are needed. The aim of the study was to develop a protocol for direct deep sequencing of high-risk (hr) HPV strains, based on the widely used commercial Hybrid Capture 2 (QIAGEN) test, without any additional probe design. This protocol was applied to 15 HPV-positive and two HPV-negative cervical samples or cell lines and validated at the genotype level by comparing the sequencing results to those obtained using a commercial genotyping kit. The performance of our protocol, presented in this proof-of-principle study, supports its use for accurate characterization of genetic variants of hrHPV.

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