Open AccessOptimized nested PCR enhances biological diagnosis and phylogenetic analysis of human parvovirus B19 infections
Author(s) -
Maxime Pichon,
Clément Labois,
Véronique Tardy-Guidollet,
Delphine Mallet,
JeanSébastien Casalegno,
Geneviève Billaud,
Bruno Lina,
P. Gaucherand,
Yahia Mekki
Publication year - 2019
Publication title -
archives of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.943
H-Index - 110
eISSN - 1432-8798
pISSN - 0304-8608
DOI - 10.1007/s00705-019-04368-w
Subject(s) - biology , virology , nested polymerase chain reaction , medical microbiology , genotype , parvovirus , phylogenetic tree , polymerase chain reaction , genetics , virus , gene
Diagnosis and epidemiological analysis of human parvovirus B19 (hB19V) infections are essential for disease management in severely ill patients. This study aimed to evaluate the performance of an optimized NS1-VP1u nested PCR for detection and sequencing of viruses in clinical samples using 224 clinical and five reference samples. PCR sensitivity, specificity, and positive and negative predictive values were perfect (100%). While phylogenetic analysis of a 615 bp-long fragment demonstrated that the viruses in all of the samples belonged to genotype 1, this study confirmed that this optimized PCR could detect all known hB19V with high performance.
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