Characterization of an Australian isolate of taro bacilliform virus and development of an infectious clone
Author(s) -
Dawit Kidanemariam,
Amit C. Sukal,
Kathy Crew,
Grahame V Jackson,
Adane Abraham,
J. L. Dale,
R. M. Harding,
Anthony P. James
Publication year - 2018
Publication title -
archives of virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.943
H-Index - 110
eISSN - 1432-8798
pISSN - 0304-8608
DOI - 10.1007/s00705-018-3783-0
Subject(s) - colocasia esculenta , biology , virology , clone (java method) , virus , inoculation , medical microbiology , araceae , gene , botany , genetics , horticulture
The badnavirus taro bacilliform virus (TaBV) has been reported to infect taro (Colocasia esculenta L.) and other edible aroids in several South Pacific island countries, but there are no published reports from Australia. Using PCR and RCA, we identified and characterized an Australian TaBV isolate. A terminally redundant cloned copy of the TaBV genome was generated and shown to be infectious in taro following agro-inoculation. This is the first report of TaBV from Australia and also the first report of an infectious clone for this virus.
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