Molecular cloning, and characterization and expression of dihydrolipoamide acetyltransferase component of murine pyruvate dehydrogenase complex in bile duct cancer cells | Zendy

Liqun Wang | Zendy; Shuichi Kaneko | Zendy; Makiko Kagaya | Zendy; Hideki Ohno | Zendy; Masao Honda | Zendy; Kenichi Kobayashi | Zendy

Open Access

Molecular cloning, and characterization and expression of dihydrolipoamide acetyltransferase component of murine pyruvate dehydrogenase complex in bile duct cancer cells

Author(s) -

Liqun Wang,

Shuichi Kaneko,

Makiko Kagaya,

Hideki Ohno,

Masao Honda,

Kenichi Kobayashi

Publication year - 2002

Publication title -

journal of gastroenterology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.33

H-Index - 114

eISSN - 1435-5922

pISSN - 0944-1174

DOI - 10.1007/s005350200065

Subject(s) - microbiology and biotechnology , biology , complementary dna , gene expression , gene , biochemistry

The association between the dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex (PDC) and primary biliary cirrhosis (PBC) is clinically established. However, the detailed pathological function of the PDC-E2 gene is as yet unclear. In order to study the gene function in knockout and transgenic mouse models, we cloned and characterized the mouse PDC-E2 (mPDC-E2) gene. Because the expression level of PDC-E2 was elevated in PBC bile duct cells, we tried to construct a bile duct carcinoma cell line that overexpressed PDC-E2 as a PBC cell model.

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