Immunolocalization of the exocytosis-sensitive phosphoprotein, PP63/parafusin, in Paramecium cells using antibodies against recombinant protein | Zendy

Roland Kissmehl | Zendy; Karin Hauser | Zendy; Markus Gößringer | Zendy; Massoud Momayezi | Zendy; Norbert Klauke | Zendy; Helmut Plattner | Zendy

Open Access

Immunolocalization of the exocytosis-sensitive phosphoprotein, PP63/parafusin, in Paramecium cells using antibodies against recombinant protein

Author(s) -

Roland Kissmehl,

Karin Hauser,

Markus Gößringer,

Massoud Momayezi,

Norbert Klauke,

Helmut Plattner

Publication year - 1998

Publication title -

histochemistry and cell biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.107

H-Index - 98

eISSN - 1432-119X

pISSN - 0948-6143

DOI - 10.1007/s004180050258

Subject(s) - exocytosis , paramecium , immunolabeling , organelle , recombinant dna , phosphoprotein , microbiology and biotechnology , ectodomain , cell fractionation , biology , chemistry , antibody , biochemistry , membrane , receptor , immunology , immunohistochemistry , phosphorylation , gene

We have localized a structure-bound fraction of the exocytosis-sensitive phosphoprotein, PP63/parafusin (PP63/pf), in Paramecium cells by widely different methods. We combined cell fractionation, western blots, as well as light and electron microscopy (pre- and post-embedding immunolabeling), applying antibodies against the recombinant protein. PP63/pf is considerably enriched in certain cortical structures, notably the outlines of regular surface fields (kinetids), docking sites of secretory organelles (trichocysts) and the membranes of subplasmalemmal Ca2+-stores (alveolar sacs). From our localization studies we tentatively derive several potential functions for PP63/pf, including cell surface structuring, assembly of exocytosis sites, and/or Ca2+ homeostasis.

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