Open AccessEffect of two different preparations of platelet‐rich plasma on synoviocytes
Author(s) -
Assirelli Elisa,
Filardo Giuseppe,
Mariani Erminia,
Kon Elizaveta,
Roffi Alice,
Vaccaro Franca,
Marcacci Maurilio,
Facchini Andrea,
Pulsatelli Lia
Publication year - 2015
Publication title -
knee surgery, sports traumatology, arthroscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.806
H-Index - 125
eISSN - 1433-7347
pISSN - 0942-2056
DOI - 10.1007/s00167-014-3113-3
Subject(s) - hepatocyte growth factor , proinflammatory cytokine , platelet rich plasma , hyaluronic acid , chemistry , platelet , growth factor , interleukin 8 , fibroblast growth factor , gene expression , endocrinology , tissue inhibitor of metalloproteinase , fibroblast , medicine , platelet derived growth factor receptor , interleukin , matrix metalloproteinase , inflammation , cytokine , biology , in vitro , gene , biochemistry , receptor , genetics
Purpose To analyse the modifications induced by two different platelet‐rich plasma (PRP) preparations on osteoarthritis (OA) synoviocytes, by documenting changes in gene expression of factors involved in joint physiopathology. Methods OA synoviocytes were cultured for 7 days in medium with different concentrations of either P‐PRP (a pure platelet concentrate without leucocytes but with a limited number of platelets), L‐PRP (a higher platelet concentrate containing leucocytes) or platelet‐poor plasma (PPP). Gene expression of interleukin (IL)‐1beta, IL‐6, IL‐8/CXCL8, tumour necrosis factor alpha, IL‐10, IL‐4, IL‐13, metalloproteinase‐13, tissue inhibitor of metalloproteinase (TIMP)‐1, (TIMP)‐3, (TIMP)‐4, vascular endothelial growth factor, transforming growth factor beta1, fibroblast growth factor (FGF)‐2, hepatocyte growth factor (HGF), hyaluronic acid (HA) synthases (HAS)‐1, (HAS)‐2, and (HAS)‐3 was analysed by RT‐PCR. HA production was determined in culture supernatants by ELISA. Results IL‐1β, IL‐8 and FGF‐2 were significantly induced by L‐PRP compared to both P‐PRP and PPP; HGF was down‐modulated by L‐PRP versus both P‐PRP and PPP, and an inverse dose–response influence was shown for all preparations. Expression level of TIMP‐4 was lower in the presence of L‐PRP compared with P‐PRP. HA production and HAS gene expression did not seem to be modulated by PRP. Conclusions L‐PRP is able to sustain the up‐regulation of proinflammatory factors, (IL‐1beta, IL‐8 and FGF‐2), together with a down‐modulation of HGF and TIMP‐4 expression, two factors that have been recognized as anti‐catabolic mediators in cartilage, thus supporting the need to further optimize the PRP preparations to be applied in clinical practice.