Open AccessA genetic linkage map of Nicotiana plumbaginifolia/ Nicotiana longiflora based on RFLP and RAPD markers
Author(s) -
TungYi Lin,
Y. Y. Kao,
SeyEn Lin,
R. F. Lin,
C. M. Chen,
ChiHsiang Huang,
C. K. Wang,
Y. Z. Lin,
C. C. Chen
Publication year - 2001
Publication title -
theoretical and applied genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.922
H-Index - 179
eISSN - 1432-2242
pISSN - 0040-5752
DOI - 10.1007/s001220100618
Subject(s) - rapd , biology , nicotiana , restriction fragment length polymorphism , genetics , genome , genetic linkage , genetic marker , linkage (software) , plant biochemistry , gene mapping , chromosome , solanaceae , gene , genetic diversity , polymerase chain reaction , population , demography , sociology
[[abstract]]We have constructed a genetic linkage map for Nicotiana plumbaginifolia/Nicotiana longiflora (2n= 2x=20), based on the segregation of 69 RFLP and 102 RAPD loci in 99 F-2 plants from the cross N. plumbaginifolia x N. longiflora. The map consists of nine major linkage groups, each containing more than nine marker loci, and spans 1062 cM. Twenty of the RFLP markers were mapped previously to Nicotiana sylvestris (2n=2x=24) chromosomes using monosomic alien addition lines. Taxonomically, N. plumbaginifolia and N. sylvestris belong to the same section, namely the Alatae; however, cytogenetic evidence indicates that they are not closely related. Comparison of the distribution of markers common to both maps suggests that genome reorganization has occurred during the evolution of these two species. Evidence is also presented that genome reorganization may be accompanied by gain and loss of specific classes of DNA sequences in their genomes.[[fileno]]2050133010006[[department]]生科
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