Open AccessHighly fluorescing solid DNA-cationic polyelectrolyte complexes prepared from a natural DNA and a poly(fluorenevinylene-alt-phenylene) bearing quaternary ammonium pendants
Author(s) -
YoungJun Yu,
Young-Wan Kwon,
Kyu Nam Kim,
Eui-Doo Do,
Dong-Hoon Choi,
Jung-Il Jin,
Hee-Won Shin,
Yong-Rok Kim,
Ik Joong Kang,
John A. Mikroyannidis
Publication year - 2009
Publication title -
macromolecular research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.491
H-Index - 49
eISSN - 2092-7673
pISSN - 1598-5032
DOI - 10.1007/bf03218687
Subject(s) - polyelectrolyte , dna , ammonium , cationic polymerization , polymer chemistry , materials science , fluorescence , polymer , nanochemistry , copolymer , phenylene , chemistry , organic chemistry , nanotechnology , biochemistry , physics , quantum mechanics , composite material
A fluorescing, copolymer (Q)-bearing, quaternary ammonium pendant was mixed with excess natural salmon sperm DNA with a molecular weight of 1.3×10 (2,000 base pairs) to afford highly fluorescing, complex mixtures. The fluorescence life-time of the polymer Q was greatly increased when mixed with DNA: for the mixture of Q:DNA=1:750 the fast and slow decay lifetimes increased from ca. 10 to 100 ps and from 20 ps to ca. 1 ns, respectively. The enhanced fluorescence of the mixtures was ascribed to efficient compartmentalization and reduced conformational relaxation of the polymer Q by complexation with excess DNA.
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