Determination of ruthenium on DNA by XRF | Zendy

L. Wielopolski | Zendy; R. Zhang | Zendy; Michael J. Clarke | Zendy; S.H. Cohn | Zendy

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Determination of ruthenium on DNA by XRF

Author(s) -

L. Wielopolski,

R. Zhang,

Michael J. Clarke,

S.H. Cohn

Publication year - 1987

Publication title -

biological trace element research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.649

H-Index - 80

eISSN - 1559-0720

pISSN - 0163-4984

DOI - 10.1007/bf02796639

Subject(s) - differential pulse voltammetry , ruthenium , atomic absorption spectroscopy , fluorescence , chemistry , analytical chemistry (journal) , absorption (acoustics) , x ray fluorescence , absorption spectroscopy , spectroscopy , fluorescence spectroscopy , cyclic voltammetry , electrochemistry , chromatography , optics , electrode , physics , biochemistry , quantum mechanics , catalysis

An X-ray fluorescence (XRF) technique is used to quantitate the binding of [H2O(NH3)5Ru(II)](2+) to DNA. This method is shown to be more sensitive, precise, and convenient than conventional optical absorption (OA) spectroscopy, differential pulse voltammetry (DPV), or atomic absorption (AA) techniques. X-ray fluorescence is insensitive to the oxidation state or coordination environment of Ru and so can be used to determine total Ru. The minimum detectable amount of Ru is 10 ng in 1 h of counting time, using a 100-mCi(125)I source. The specific advantages of the XRF method over the conventional methods are outlined.

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