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Summary Experiments have been performed in batch fermentation, using a defined medium, to investigate the effects of phosphate and ammonium ion concentrations on solvent production usingClostridium acetobutylicum. Solvent production occurred under conditions of either ammonium- or phosphate-limitation, but the optimum conditions were observed to be where both of these nutrients were slightly in excess of growth requirements. A greater excess of nutrients caused the fermentation to be acidogenic rather than solventogenic.

Effect of phosphate and ammonium ion concentrations on solvent production in defined medium byClostridium acetobutylicum