Sequence of the gene encoding the 16S rRNA of the beer spoilage organismMegasphaera cerevisiae
Author(s) -
Liam Doyle,
James O. McInerney,
Jennifer Mooney,
R. Powell,
Auli Haikara,
Anthony P. Moran
Publication year - 1995
Publication title -
journal of industrial microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.857
H-Index - 112
ISSN - 0169-4146
DOI - 10.1007/bf01569801
Subject(s) - 16s ribosomal rna , biology , gene , phylogenetic tree , ribosomal rna , organism , polymerase chain reaction , food spoilage , microbiology and biotechnology , genetics , bacteria
The 16S ribosomal RNA gene from the beer-spoilage organism, Megasphaera cerevisiae was polymerase chain reaction (PCR)-amplified and sequenced. Analysis confirmed the phylogenetic position of M. cerevisiae as a sister taxon of Megasphaera elsdenii, within the obligately anaerobic, Gram-negative cocci. The sequence obtained should facilitate the development of DNA probes for early detection of this spoilage organism.
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