Regioselectivity enhancement by partial purification of lipase fromAspergillus niger | Zendy

HaoPing Chen | Zendy; KwoFeng Hsiao | Zendy; ShihHsiung Wu | Zendy; KungTsung Wang | Zendy

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Regioselectivity enhancement by partial purification of lipase fromAspergillus niger

Author(s) -

HaoPing Chen,

KwoFeng Hsiao,

ShihHsiung Wu,

KungTsung Wang

Publication year - 1995

Publication title -

biotechnology letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.548

H-Index - 107

eISSN - 1573-6776

pISSN - 0141-5492

DOI - 10.1007/bf01190643

Subject(s) - lipase , aspergillus niger , yield (engineering) , chemistry , regioselectivity , fraction (chemistry) , triacylglycerol lipase , chromatography , ion exchange resin , organic chemistry , enzyme , biochemistry , catalysis , materials science , metallurgy

Lipase from was partially purified by a single step of ion-exchange chromatography and two major fractions I and II could be obtained. Two substrates, peracetylated 1-methyl and 1-thioethyl β-D-glucopyranosides, were deacetylated by fraction II to give 3-OH derivatives as sole products with very high yield, respectively.

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