Probe walking: development of novel probes for DNA fingerprinting | Zendy

Keiko Washio | Zendy; Shogo Misawa | Zendy; Shintaroh Ueda | Zendy

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Probe walking: development of novel probes for DNA fingerprinting

Author(s) -

Keiko Washio,

Shogo Misawa,

Shintaroh Ueda

Publication year - 1989

Publication title -

human genetics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.351

H-Index - 137

eISSN - 1432-1203

pISSN - 0340-6717

DOI - 10.1007/bf00285160

Subject(s) - biology , hypervariable region , genetics , tandem repeat , dna , dna profiling , hybridization probe , variable number tandem repeat , molecular probe , repeated sequence , dna sequencing , base pair , restriction fragment , microbiology and biotechnology , gene , genome , allele

The tandem repeat of a 28-base-pair (bp) sequence downstream of the human c-Ha-ras-1 oncogene was studied as a probe for DNA fingerprinting. Multiple hypervariable patterns were observed by Southern hybridization at low stringency. The patterns were specific to individuals, indicating the availability of the 28-bp repeat as a probe for DNA fingerprinting. Moreover, we cloned the tandem repeat of a 33-bp sequence, which cross-hybridized with the 28-bp repeat. This 33-bp repeat detected another set of hypervariable restriction fragments by Southern hybridization at the same stringency. These results suggests that 'probe walking' can be employed to develop novel probes that provide different DNA fingerprints.

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