Open AccessProteins of seminal fluid and spermatozoa in the trout (Oncorhynchus mykiss): Partial characterization and variations
Author(s) -
Maurice Loir,
Catherine Labbé,
Gérard Maisse,
Alain Pinson,
Geneviève Boulard,
Brigitte Mourot,
F. Chambeyron
Publication year - 1990
Publication title -
fish physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 79
eISSN - 1573-5168
pISSN - 0920-1742
DOI - 10.1007/bf00003405
Subject(s) - biology , sperm , rainbow trout , immunoelectrophoresis , polyacrylamide gel electrophoresis , electrophoresis , semen , gel electrophoresis , blood proteins , andrology , biochemistry , antibody , immunology , anatomy , fish <actinopterygii> , genetics , fishery , medicine , enzyme
The protein composition of seminal fluid, blood serum, sperm plasma membrane and flagellum of rainbow trout were analysed by SDS-polyacrylamide gel electrophoresis. Immunological identity between proteins of the 2 fluids and sperm components was studied using crossed immunoelectrophoresis, rocket immunoelectrophoresis and immunoblotting. Results indicate that many seminal proteins are antigenically-related to serum proteins, proteins of sperm origin are present in seminal fluid in varying amounts, depending on the animals and sampling time, and several serum-like seminal proteins are bound to spermatozoa.Lipoproteins were isolated from seminal fluid (mean level: 33 μg/ml) and characterized. They were identified as being HDL-like lipoproteins. A possible physiological role is proposed for these seminal lipoproteins.
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