Differentiation of Adipocytes in Monolayer from Mouse Embryonic Stem Cells | Zendy

Ixchelt CuarantaMonroy | Zendy; Zoltán Simándi | Zendy; László Nagy | Zendy

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Differentiation of Adipocytes in Monolayer from Mouse Embryonic Stem Cells

Author(s) -

Ixchelt CuarantaMonroy,

Zoltán Simándi,

László Nagy

Publication year - 2015

Publication title -

methods in molecular biology

Language(s) - English

Resource type - Book series

SCImago Journal Rank - 0.711

H-Index - 152

eISSN - 1940-6029

pISSN - 1064-3745

DOI - 10.1007/7651_2015_219

Subject(s) - embryonic stem cell , adipocyte , induced pluripotent stem cell , microbiology and biotechnology , stem cell , biology , cellular differentiation , adipose tissue , endocrinology , genetics , gene

Obesity and its comorbidity incidence have increased worldwide during the past 10 years. In consequence, researchers have drawn their attention to the understanding of adipocyte differentiation. Several cellular model systems have been established; however no efficient protocol could be developed so far to differentiate the pluripotent embryonic stem cells to adipocytes. In this chapter, we describe a detailed protocol that is optimized for mouse embryonic stem cells. The result of this differentiation is a homogenous adipocyte monolayer culture that can be used for several applications including developmental and pharmacological research.

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