Open AccessIntegrated transcriptomic, phenotypic, and functional study reveals tissue‐specific immune properties of mesenchymal stromal cells
Author(s) -
Ménard Cédric,
Dulong Joëlle,
Roulois David,
Hébraud Benjamin,
Verdière Léa,
Pangault Céline,
Sibut Vonick,
Bezier Isabelle,
Bescher Nadège,
Monvoisin Céline,
Gadelorge Mélanie,
Bertheuil Nicolas,
Flécher Erwan,
Casteilla Louis,
Collas Philippe,
Sensebé Luc,
Bourin Philippe,
Espagnolle Nicolas,
Tarte Karin
Publication year - 2020
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.3077
Subject(s) - mesenchymal stem cell , biology , adipose tissue , stromal cell , immune system , bone marrow , transcriptome , phenotype , immunogenicity , immunology , microbiology and biotechnology , gene expression , gene , cancer research , genetics , endocrinology
Clinical‐grade mesenchymal stromal cells (MSCs) can be expanded from bone marrow and adipose tissue to treat inflammatory diseases and degenerative disorders. However, the influence of their tissue of origin on their functional properties, including their immunosuppressive activity, remains unsolved. In this study, we produced paired bone marrow‐derived mesenchymal stromal cell (BM‐MSC) and adipose‐derived stromal cell (ASC) batches from 14 healthy donors. We then compared them using transcriptomic, phenotypic, and functional analyses and validated our results on purified native MSCs to infer which differences were really endowed by tissue of origin. Cultured MSCs segregated together owing to their tissue of origin based on their gene expression profile analyzed using differential expression and weighted gene coexpression network analysis. This translated into distinct immune‐related gene signatures, phenotypes, and functional cell interactions. Importantly, sorted native BM‐MSCs and ASCs essentially displayed the same distinctive patterns than their in vitro‐expanded counterparts. As a whole, ASCs exhibited an immune profile consistent with a stronger inhibition of immune response and a lower immunogenicity, supporting the use of adipose tissue as a valuable source for clinical applications.