Open AccessIGF1 Promotes Adipogenesis by a Lineage Bias of Endogenous Adipose Stem/Progenitor Cells
Author(s) -
Hu Li,
Yang Guodong,
Hägg Daniel,
Sun Guoming,
Ahn Jeffrey M.,
Jiang Nan,
Ricupero Christopher L.,
Wu June,
Rodhe Christine Hsu,
Ascherman Jeffrey A.,
Chen Lili,
Mao Jeremy J.
Publication year - 2015
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.2052
Subject(s) - adipogenesis , biology , adipose tissue , wnt signaling pathway , stromal vascular fraction , stem cell , axin2 , microbiology and biotechnology , stromal cell , progenitor cell , endocrinology , medicine , cancer research , signal transduction
Adipogenesis is essential for soft tissue reconstruction following trauma or tumor resection. We demonstrate that CD31 − /34 + /146 − cells, a subpopulation of the stromal vascular fraction (SVF) of human adipose tissue, were robustly adipogenic. Insulin growth factor‐1 (IGF1) promoted a lineage bias towards CD31 − /34 + /146 − cells at the expense of CD31 − /34 + /146 + cells. IGF1 was microencapsulated in poly(lactic‐ co ‐glycolic acid) scaffolds and implanted in the inguinal fat pad of C57Bl6 mice. Control‐released IGF1 induced remarkable adipogenesis in vivo by recruiting endogenous cells. In comparison with the CD31 − /34 + /146 + cells, CD31 − /34 + /146 − cells had a weaker Wnt/β‐catenin signal. IGF1 attenuated Wnt/β‐catenin signaling by activating Axin2/PPARγ pathways in SVF cells, suggesting IGF1 promotes CD31 − /34 + /146 − bias through tuning Wnt signal. PPARγ response element (PPRE) in Axin2 promoter was crucial for Axin2 upregulation, suggesting that PPARγ transcriptionally activates Axin2 . Together, these findings illustrate an Axin2/PPARγ axis in adipogenesis that is particularly attributable to a lineage bias towards CD31 − /34 + /146 − cells, with implications in adipose regeneration. S tem C ells 2015;33:2483–2495