Open AccessLoss of Faap20 Causes Hematopoietic Stem and Progenitor Cell Depletion in Mice Under Genotoxic Stress
Author(s) -
Zhang Tingting,
Wilson Andrew F.,
Mahmood Ali Abdullah,
Namekawa Satoshi H.,
Andreassen Paul R.,
Ruhikanta Meetei Amom,
Pang Qishen
Publication year - 2015
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.2048
Subject(s) - fanca , biology , fanconi anemia , progenitor cell , haematopoiesis , stem cell , fancd2 , microbiology and biotechnology , cancer research , bone marrow failure , dna repair , genetics , gene
20‐kDa FANCA‐associated protein (FAAP20) is a recently identified protein that associates with the Fanconi anemia (FA) core complex component, FANCA. FAAP20 contains a conserved ubiquitin‐binding zinc‐finger domain and plays critical roles in the FA‐BRCA pathway of DNA repair and genome maintenance. The function of FAAP20 in animals has not been explored. Here, we report that deletion of Faap20 in mice led to a mild FA‐like phenotype with defects in the reproductive and hematopoietic systems. Specifically, hematopoietic stem and progenitor cells (HSPCs) from Faap20 − / − mice showed defects in long‐term multilineage reconstitution in lethally irradiated recipient mice, with milder phenotype as compared to HSPCs from Fanca − / − or Fancc − / − mice. Faap20 − / − mice are susceptible to mitomycin C (MMC)‐induced pancytopenia. That is, acute MMC stress induced a significant progenitor loss especially the erythroid progenitors and megakaryocyte–erythrocyte progenitors in Faap20 − / − mice. Furthermore, Faap20 − / − HSPCs displayed aberrant cell cycle pattern during chronic MMC treatment. Finally, using Faap20 − / − Fanca − / − double‐knockout mice, we demonstrated a possible dominant effect of FANCA in the interaction between FAAP20 and FANCA. This novel Faap20 mouse model may be valuable in studying the regulation of the FA pathway during bone marrow failure progress in FA patients. S tem C ells 2015;33:2320–2330