Open AccessConcise Review: Understanding Clonal Dynamics in Homeostasis and Injury Through Multicolor Lineage Tracing
Author(s) -
Roy Edwige,
Neufeld Zoltan,
Livet Jean,
Khosrotehrani Kiarash
Publication year - 2014
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.1804
Subject(s) - biology , stem cell , lineage (genetic) , tracing , identification (biology) , evolutionary biology , computational biology , cell type , developmental biology , microbiology and biotechnology , genetics , cell , gene , ecology , computer science , operating system
A bstract Lineage tracing is an essential tool to study stem cell fate. Although traditional lineage tracing techniques have considerably advanced our understanding of stem cell behavior, they pose significant limitations for identification and longitudinal tracking of the progeny of individual stem cells, to compare their behaviors. This is of importance given the well‐established heterogeneity among stem cells both in terms of potentialities and proliferative capacities. The recent development of multicolor genetic reporters addressable to specific cell populations largely overcomes these issues. These new “rainbow” technologies provide increased resolution in clonal identification and offer the possibility to study the relative distribution, contacts, tiled arrangement, and competitive interactions among cells or groups of cells of the same type. S tem C ells 2014;32:3046–3054