Open AccessExpression of Murine Interleukin 11 and its Receptor α‐Chain in Adult and Embryonic Tissues
Author(s) -
Davidson Alan J.,
Freeman SallyAnne,
Crosier Kathryn E.,
Wood Clive R.,
Crosier Philip S.
Publication year - 1997
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.150119
Subject(s) - biology , embryonic stem cell , microbiology and biotechnology , embryoid body , haematopoiesis , stem cell , embryogenesis , embryo , adult stem cell , gene , genetics
Interleukin 11 (IL‐11) is a multifunctional cytokine that has diverse effects on blood cells and their precursors and on a number of cell types outside of the hematopoietic system. The cDNAs encoding murine IL‐11 and its receptor α‐chain (IL‐11Rα) have recently been isolated. We have used the RNase protection assay to examine the expression of murine IL‐11 and IL‐11Rα in a range of adult mouse tissues, in embryos, and during development of embryonic stem (ES) cells into cystic embryoid bodies in vitro. The testis showed a high level of IL‐11 gene expression while a much lower level of expression was detected in the lung, stomach, small intestine, and large intestine. Expression of IL‐11 was not detected between day 10.5 and day 18.5 post coitum of embryonic development or in differentiating ES cells in vitro. In contrast, the IL‐11Rα was found to be expressed in all adult tissues examined, during embryonic development, and in totipotent and differentiating ES cells.