Open AccessProangiogenic and Prosurvival Functions of Glucose in Human Mesenchymal Stem Cells upon Transplantation
Author(s) -
Deschepper Mickael,
Manassero Mathieu,
Oudina Karim,
Paquet Joseph,
Monfoulet LaurentEmmanuel,
Bensidhoum Morad,
LogeartAvramoglou Delphine,
Petite Herve
Publication year - 2013
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.1299
Subject(s) - mesenchymal stem cell , biology , transplantation , stem cell , microbiology and biotechnology , in vitro , in vivo , cell , downregulation and upregulation , medicine , biochemistry , gene
A major limitation in the development of cellular therapies using human mesenchymal stem cells (hMSCs) is cell survival post‐transplantation. In this study, we challenged the current paradigm of hMSC survival, which assigned a pivotal role to oxygen, by testing the hypothesis that exogenous glucose may be key to hMSC survival. We demonstrated that hMSCs could endure sustained near‐anoxia conditions only in the presence of glucose. In this in vitro cell model, the protein expressions of Hif‐1α and angiogenic factors were upregulated by the presence of glucose. Ectopically implanted tissue constructs supplemented with glucose exhibited four‐ to fivefold higher viability and were more vascularized compared to those without glucose at day 14. These findings provided the first direct in vitro and in vivo demonstration of the proangiogenic and prosurvival functions of glucose in hMSC upon transplantation and identified glucose as an essential component of the ideal scaffold for transplanting stem cells. S TEM C ELLS 2013;31:526–535