Open AccessLimited Gene Expression Variation in Human Embryonic Stem Cell and Induced Pluripotent Stem Cell‐Derived Endothelial Cells
Author(s) -
White Mark P.,
Rufaihah Abdul J.,
Liu Lei,
Ghebremariam Yohannes T.,
Ivey Kathryn N.,
Cooke John P.,
Srivastava Deepak
Publication year - 2013
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.1267
Subject(s) - biology , embryonic stem cell , induced pluripotent stem cell , stem cell , endothelial stem cell , microbiology and biotechnology , gene , gene expression , cell , genetics , in vitro
Recent evidence suggests human embryonic stem cell (hESC) and induced pluripotent stem (iPS) cell lines have differences in their epigenetic marks and transcriptomes, yet the impact of these differences on subsequent terminally differentiated cells is less well understood. Comparison of purified, homogeneous populations of somatic cells derived from multiple independent human iPS and ES lines will be required to address this critical question. Here, we report a differentiation protocol based on embryonic development that consistently yields large numbers of endothelial cells (ECs) derived from multiple hESCs or iPS cells. Mesoderm differentiation of embryoid bodies was maximized, and defined growth factors were used to generate KDR + EC progenitors. Magnetic purification of a KDR + progenitor subpopulation resulted in an expanding, homogeneous pool of ECs that expressed EC markers and had functional properties of ECs. Comparison of the transcriptomes revealed limited gene expression variability between multiple lines of human iPS‐derived ECs or between lines of ES‐ and iPS‐derived ECs. These results demonstrate a method to generate large numbers of pure human EC progenitors and differentiated ECs from pluripotent stem cells and suggest individual lineages derived from human iPS cells may have significantly less variance than their pluripotent founders. S TEM C ells 2013;31:92–103