Open AccessCell Autonomous and Nonautonomous Mechanisms Drive Hematopoietic Stem/progenitor Cell Loss in the Absence of DNA Repair
Author(s) -
Cho Joon Seok,
Kook Sung Ho,
Robinson Andria Rasile,
Niedernhofer Laura J.,
Lee ByeongChel
Publication year - 2013
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1002/stem.1261
Subject(s) - biology , progenitor cell , stem cell , microbiology and biotechnology , dna damage , haematopoiesis , bone marrow , dna repair , immunology , senescence , ercc1 , hematopoietic stem cell , cancer research , nucleotide excision repair , dna , genetics
Daily, cells incur tens of thousands of DNA lesions caused by endogenous processes. Due to their long‐lived nature, adult stem cells may be particularly susceptible to the negative impact of this constant genotoxic stress. Indeed, in murine models of DNA repair deficiencies, there is accumulation of DNA damage in hematopoietic stem cells and premature loss of function. Herein, we demonstrate that mice expressing reduced levels of ERCC1‐XPF DNA repair endonuclease ( Ercc1 −/Δ mice) spontaneously display a progressive decline in the number and function of hematopoietic stem/progenitor cells (HSPCs). This was accompanied by increased cell death, expression of senescence markers, reactive oxygen species, and DNA damage in HSPC populations, illustrating cell autonomous mechanisms that contribute to loss of function. In addition, the bone marrow microenvironment of Ercc1 −/Δ mice was not permissive for the engraftment of transplanted normal stem cells. Bones from Ercc1 −/Δ mice displayed excessive osteoclastic activity, which alters the microenvironment in a way that is unfavorable to HSPC maintenance. This was accompanied by increased proinflammatory cytokines in the bone marrow of Ercc1 −/Δ mice. These data provide novel evidence that spontaneous, endogenous DNA damage, if not repaired, promotes progressive attrition of adult stem cells via both cell autonomous and nonautonomous mechanisms. S TEM C ELLS 2013;31:511–525