Open AccessPhenotypic and Functional Characterization of Müller Glia Isolated from Induced Pluripotent Stem Cell‐Derived Retinal Organoids: Improvement of Retinal Ganglion Cell Function upon Transplantation
Author(s) -
Eastlake Karen,
Wang Weixin,
Jayaram Hari,
MurrayDunning Celia,
Carr Amanda J. F.,
Ramsden Conor M.,
Vugler Anthony,
Gore Katrina,
Clemo Nadine,
Stewart Mark,
Coffey Pete,
Khaw Peng T.,
Limb G. Astrid
Publication year - 2019
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.1002/sctm.18-0263
Subject(s) - induced pluripotent stem cell , retinal , transplantation , biology , stem cell , organoid , microbiology and biotechnology , muller glia , neuroscience , retina , retinal ganglion cell , giant retinal ganglion cells , progenitor cell , medicine , embryonic stem cell , genetics , biochemistry , gene
Abstract Glaucoma is one of the leading causes of blindness, and there is an ongoing need for new therapies. Recent studies indicate that cell transplantation using Müller glia may be beneficial, but there is a need for novel sources of cells to provide therapeutic benefit. In this study, we have isolated Müller glia from retinal organoids formed by human induced pluripotent stem cells (hiPSCs) in vitro and have shown their ability to partially restore visual function in rats depleted of retinal ganglion cells by NMDA. Based on the present results, we suggest that Müller glia derived from retinal organoids formed by hiPSC may provide an attractive source of cells for human retinal therapies, to prevent and treat vision loss caused by retinal degenerative conditions. Stem Cells Translational Medicine 2019;8:775&784