Open AccessProminin‐1/CD133: Lipid Raft Association, Detergent Resistance, and Immunodetection
Author(s) -
Karbanová Jana,
Lorico Aurelio,
Bornhäuser Martin,
Corbeil Denis,
Fargeas Christine A.
Publication year - 2018
Publication title -
stem cells translational medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.781
H-Index - 71
eISSN - 2157-6580
pISSN - 2157-6564
DOI - 10.1002/sctm.17-0223
Subject(s) - lipid raft , stem cell , biology , microbiology and biotechnology , signal transduction
Summary The cell surface antigen prominin‐1 (alias CD133) has gained enormous interest in the past 2 decades and given rise to debates as to its utility as a biological stem and cancer stem cell marker. Important and yet often overlooked knowledge that is pertinent to its physiological function has been generated in other systems given its more general expression beyond primitive cells. This article briefly discusses the importance of particular biochemical features of CD133 with relation to its association with membrane microdomains (lipid rafts) and proper immunodetection. It also draws attention toward the adequate use of detergents and caveats that may apply to the interpretation of the results generated. S tem C ells T ranslational M edicine 2018;7:155–160