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Low Cost, Sensitive Impedance Detection of E. coli Bacteria in Food‐Matrix Samples Using Surface‐Imprinted Polymers as Whole‐Cell Receptors
Author(s) -
Stilman Wouter,
Campolim Lenzi Mariana,
Wackers Gideon,
Deschaume Olivier,
Yongabi Derick,
Mathijssen Glenn,
Bartic Carmen,
Gruber Jonas,
Wübbenhorst Michael,
Heyndrickx Marc,
Wagner Patrick
Publication year - 2022
Publication title -
physica status solidi (a)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 104
eISSN - 1862-6319
pISSN - 1862-6300
DOI - 10.1002/pssa.202100405
Subject(s) - detection limit , molecularly imprinted polymer , biosensor , dielectric spectroscopy , materials science , context (archaeology) , polymer , analytical chemistry (journal) , chromatography , chemistry , nanotechnology , selectivity , biochemistry , biology , composite material , electrode , electrochemistry , paleontology , catalysis
Herein, a biomimetic sensor platform that allows sensitive, onsite detection of Escherichia coli ( E. coli ) with a limit of detection of 30 cells mL −1 in both buffer suspension and rinsing water from an industrial food‐preparation machine is reported. Ultrathin surface‐imprinted polymers are combined with non‐Faradaic impedance spectroscopy to measure the increase in resistance at the solid‐to‐liquid interface due to the binding of target cells by the receptor layer. The detection limit reached with this sensing principle is determined using an established, commercial impedance spectrometer and a low‐cost, home‐built impedance unit. Cross‐selectivity tests, with both an unrelated bacterial species and four species belonging to the same Enterobacteriales order, show that the response is strongest for the target bacterium while only a small cross‐selectivity signal (≈10–25%) is visible for all other types of bacteria. Therefore, this sensor is not only fast and low cost, but also sensitive and selective. Sample preparation is minimal, which is an asset for onsite bacterial detection in a food industry context.