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On‐tissue chemical derivatization reagents for matrix‐assisted laser desorption/ionization mass spectrometry imaging
Author(s) -
Merdas Mira,
Lagarrigue Mélanie,
Vanbellingen Quentin,
Umbdenstock Thierry,
Da Violante Georges,
Pineau Charles
Publication year - 2021
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.4731
Subject(s) - chemistry , reagent , derivatization , mass spectrometry , analyte , mass spectrometry imaging , maldi imaging , matrix assisted laser desorption/ionization , sample preparation , matrix (chemical analysis) , chromatography , desorption , organic chemistry , adsorption
Matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is a key tool for the analysis of biological tissues. It provides spatial and quantitative information about different types of analytes within tissue sections. Despite the increasing improvements of this technique, the low detection sensitivity of some compounds remains an important challenge to overcome. Poor sensitivity is related to weak ionization efficiency, low abundance of analytes and matrix ions, or endogenous interferences. On‐tissue chemical derivatization (OTCD) has proven to be an important solution to these issues and is increasingly employed in MALDI MSI studies. OTCD reagents, synthesized or commercially available, have been essentially used for the detection of small exogenous or endogenous molecules within tissues. Optimally, an OTCD reaction is performed in mild conditions, in an acceptable range of time, preserves the integrity of the tissues, and prevents the delocalization. In addition to their reactivity with a targeted chemical function, some OTCD reagents can also be used as a matrix, which simplifies the sample preparation procedure. In this review, we present an exhaustive overview of OTCD reagents and methods used in MALDI MSI studies.